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OriGen PermaLife Bags
Cell Culture: The OriGen PermaLife™
bag represents the optimal way to harvest, expand, protect
and preserve critical cell cultures. The bags have received
marketing clearance from FDA and indicated for use in
protecting, storing and freezing cells and tissues.
Made completely of inert FEP, the PermaLife bag contains
no plasticizers or chemical residuals to leach into or alter
delicate cell cultures. The PermaLife cell storage
bag is commonly used in clinical applications such as cell
collection, genetic therapies, immunotherapy, vector production
and cryopreservation.
Freezing Bags: The Permalife storage bags
can also be used as freezing bags for cell suspensions.
FEP remains flexible at liquid nitrogen temperatures, and
is unsurpassed as a cryogenic container.
Uses of the Origen Permalife Bag
Cell
Storage Cell Freezing
Genetic Engineering
Vector Production
Freezing / Cryopreservation
The PermaLife FEP bags are gas permeable, so oxygen
transfer to stored cells can be easily accomplished. However,
water loss across FEP film is negligible, so water loss
is low. FEP is chemically inert and contains no plasticizer,
so delicate cells do not react with FEP.
The PermaLife FEP bag is also ideal for long-term
cell storage, because it resists freezing temperatures and
cryogenic preservation. FEP remains completely flexible
down to liquid nitrogen temperatures -196°C.
Features of the OriGen PermaLife cell storage bags:
| Inert: |
FEP is chemically and biologically inert, so the
bag will not alter the culture or the medium. FEP
neither combines with any element, nor gives up any
components of its own to the solution held in the
cell culture bag. |
| Breathable: |
FEP film is gas permeable and gas transfer rates
for oxygen, carbon dioxide and nitrogen have been
published. However, FEP is practically impermeable
to water so water losses from the cell culture is
negligible. Humidified incubators - potential sources
of mold and contamination - are not necessary. |
| Sealed: |
The PermaLife cell storage bag is a closed system,
so the possibility of contamination is greatly reduced.
Swabable, needle-free valves are available to reduce
the chance of contamination and needle sticks. |
| Optical: |
The PermaLife bag has an optical transmission of
greater than 95%. Observation of cell cultures under
a microscope is easy, and can be done without risking
contamination of the sample. |
| Thermal: |
The PermaLife bag can be used as a freezing bag,
and remains flexible with no significant decrease
in mechanical strength at temperatures from cryogenic
storage (-196ºC) to high autoclave (+137ºC). NOTE:
Because FEP is gas permeable, direct immersion of
the Permalife freezing bag in liquid nitrogen can
lead to nitrogen migration into the bag, with possible
rupture on rewarming. If direct immersion in liquid
nitrogen is anticipated, package the FEP bag in an
OriGen CryoBag. |
| Safety Features: |
Each bag has a label pocket and hanger hole. . Swabable,
needle-free luer adapter valves are included to reduce
the chance of needle sticks and sample contamination.
Cell culture bags can be autoclaved for safe disposal
after use. The FEP Permalife culture bag remains flexible
from +200ºC to -200ºC, and is very well suited to
centrifuge use. |
| Needle-Free: |
A sterile, needle-free luer adapter is attached
to each bag. This self-sealing valve makes it easy
to feed and harvest sensitive cells without having
to expose them to contamination. The valve face is
swabable and can be activated with any male luer device,
such as a standard syringe. |
| Catalog Nr. |
Volume,
nominal, ml |
Volume,
max, ml |
Ports |
Width,
cm |
Length,
cm |
Surface
Area, m2 |
O2 |
CO2 |
N2 |
H2O |
| PL07 |
7 |
12 |
1 |
3 |
8 |
0.005 |
8.4 |
135 |
26 |
0.04 |
| PL30 |
30 |
50 |
2 |
9 |
6 |
0.011 |
171 |
276 |
53 |
0.07 |
| PL70 |
70 |
145 |
2 |
9 |
10 |
0.017 |
27 |
442 |
85 |
0.12 |
| PL120 |
120 |
300 |
2 |
9 |
14 |
0.025 |
40.3 |
653 |
126 |
0.18 |
| PL240 |
240 |
900 |
2 |
14 |
19 |
0.054 |
86.5 |
1400 |
270 |
0.38 |
| PL325 |
325 |
1100 |
2 |
14 |
26 |
0.073 |
116.5 |
1885 |
364 |
0.51 |
Film Permeability Table Legend:
1.Nominal volume in ml: Freezing volume of bag at 1 cm thickness
2.Total surface area, both sides of bag
3.Gas permeability is in ml per 24 hours at 25°C (Ref: "Teflon
FEP Film" DuPont Bulletin E-80412)
4.Water transmission is in g per 24 hours at 40°C (Ref:
"Teflon FEP Film" DuPont Bulletin E-80412)
Ordering Information:
Sterile, non-pyrogenic, for single use only. Individually
packed.
Custom Configurations
Custom configurations and more are available.
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