An Optimal Way to Protect Critical Cell Cultures

Intended Use: The OriGen Biomedical® PermaLife bag is intended for use in cell culture and in protecting, storing, and freezing cells and tissues.


FDA 510(k) Cleared. CE Marked.

OriGen Biomedical sells directly and works with a network of global distributors. For availability in your country, please contact us below.

  • User Inspired Design

    A sterile, needle-free, luer-actuated adapter is attached to most bag configurations. This self-sealing valve makes it easy to feed and harvest sensitive cells.

  • Gas Permeable – Water Impermeable

    Fluoroethyl polymer (FEP) film is gas permeable and gas transfer rates for oxygen, carbon dioxide and nitrogen are well-established. However, FEP film is practically impermeable to water so water losses from the cell culture are negligible.

  • Observe Your Culture

    The PermaLife bag is transparent, allowing observation of the cell culture under a microscope without risking contamination of the culture.

  • Inert

    FEP film has no plasticizers, is chemically inert, and is biologically safe for the intended use so the bag will not alter the culture or the medium.


Interested in the PermaLife Cell Culture Bag?

Contact us today to get a price quote or sample products.

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Product Use

Product Code

Working Volume, ml



PL07-2G 10 Pouch of 1  
PL30-2G 40 Pouch of 1  
PL70-2G 100 Pouch of 1  
PL120-2G 150 Pouch of 1  
PL240-2G 300 Pouch of 1  
PL325-2G 450 Pouch of 1  
PL500P-2G 750 Pouch of 1

Special Order

PL750-2G 1000 Pouch of 1  
PL1000P-2G 1500 Pouch of 1  
PL2000P2-2G 3000 Pouch of 1  

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In cell culture - what is the benefit of using a closed system?

Bags create a closed system that reduces the possibility of contamination. Swabbable needle-free valves are provided that provide repeated sterile access (and prevent needle sticks). Our cell culture bags have a high optical transmission that allows for the inspection of contents without risking contamination of the culture. The bags are gas-permeable and provide high rates of oxygen transfer to cells under culture. Additionally, water permeability is very low, so water loss is negligible and humidified incubators are not required.

What are the dimensions of a particular PermaLife Bag?

All sizing information can be found on the product brochure.

How can I handle my PermaLife products to help prevent undesired outcomes from occurring during use?

Please download our Handling Guidelines for Optimum Use When Using Your OriGen Biomedical PermaLife Cell Culture Bag here.

What is the expiration date of PermaLife Products?

4 years

I have a problem with a product, what is the complaint procedure?

Please report it as soon as possible. Contact the OriGen head office or your sales representative. Please retain the product and its packaging as this will help us in our investigation and findings. All complaints will receive a written or oral response acknowledging receipt of the product and initial findings.

Where can I find the Instructions for Use (IFU) or Certificate of Conformance (CoC) for the product I received?

An IFU and CoC will be in your original shipment box. If these items are misplaced, you can access the IFU and CoC for your OriGen products through the Client Login. If you do not have a current login, you will need to register for an account through that page.

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Nelson RC, Fellowes V, Jin P, et al. Group O plasma as a media supplement for CAR-T cells and other adoptive T-cell therapies. Transfusion. 2020 May;60(5):1004-1014. DOI: 10.1111/trf.15745. PMID: 32167176; PMCID: PMC8404615.

Klemm F, Maas RR, Bowman RL, et al. Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells. Cell. 2020 Jun;181(7):1643-1660.e17. DOI: 10.1016/j.cell.2020.05.007. PMID: 32470396; PMCID: PMC8558904.

Chen 陈温纯 W, Voos KM, Josephson CD, Li R. Short-Acting Anti-VWF (von Willebrand Factor) Aptamer Improves the Recovery, Survival, and Hemostatic Functions of Refrigerated Platelets. Arteriosclerosis, Thrombosis, and Vascular Biology. 2019 Oct;39(10):2028-2037. DOI: 10.1161/atvbaha.119.312439. PMID: 31315441; PMCID: PMC6761020.

Jin J, Gkitsas N, Fellowes VS, et al. Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules. Journal of Translational Medicine. 2018 Jan;16(1):13. DOI: 10.1186/s12967-018-1384-z. PMID: 29368612; PMCID: PMC5784598.

Fekete N, Béland AV, Campbell K, Clark SL, Hoesli CA. Bags versus flasks: a comparison of cell culture systems for the production of dendritic cell-based immunotherapies. Transfusion. 2018 Jul;58(7):1800-1813. DOI: 10.1111/trf.14621. PMID: 29672857.

Special order items will always be manufactured to order – plan for minimum quantities and extended lead times.

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