Phase 1: Cell Isolation and Separation
The first step in tissue engineering is isolating cells from donor tissue or the patient. This process requires precision to ensure cell viability and purity.
The OriGen Biomedical CryoStore Freezing Bags, designed for freezing blood and blood components, excel at maintaining cell viability during cryopreservation. They come in multiple sizes to accommodate various cell quantities and are compatible with controlled-rate freezers for optimal cryopreservation. While effective for freezing, CryoStore is not intended to store isolated cells outside of this process.
Additionally, OriGen offers O-Wrap® Bags as secondary containers for cryobags during long-term frozen storage. O-Wrap Bags are made of EVO copolymer, which remains flexible at liquid nitrogen temperatures, and feature excellent optical clarity for easy inspection of contents.
Phase 2: Cell Culture and Expansion
Once isolated, cells need to be cultured and expanded to achieve sufficient numbers for tissue construction. This phase requires a controlled environment that promotes cell growth and proliferation.
OriGen Biomedical offers PermaLife® Cell Culture Bags. These bags create an ideal environment for cell proliferation, featuring gas-permeable FEP material for efficient oxygen and CO2 exchange, excellent optical clarity for easy cell monitoring, and a closed-system design.
PermaLife® Cell Culture Bag
Phase 3: Scaffold Seeding
Expanded cells are introduced to scaffolds, which provide a structural framework for tissue growth. This process requires precision and sterility to ensure proper cell distribution.
OriGen Biomedical Accessory Sets Syringes, primarily designed for fluid transfer, can support this phase by introducing cell suspensions onto scaffolds. These syringes can aid in the application of cell-containing fluids during the seeding process.
Phase 4: Tissue Maturation
After seeding, the cell-scaffold constructs are cultured to allow for tissue maturation. This phase often involves using bioreactors to provide the necessary environmental cues for tissue development.
OriGen Biomedical provides PermaLife® Mid-to-Large Cell Culture Bags that are suitable for scaling up tissue constructs. These larger bags may aid in tissue maturation, featuring gas-permeable FEP material for efficient oxygen and CO2 exchange.
Phase 5: Harvesting Engineered Tissues
Once the engineered tissues have matured, they need to be harvested and prepared for application or further processing. This step requires careful handling to maintain tissue integrity and sterility.
OriGen Biomedical offers Manifold Sets designed for sterile fluid transfer, which can be utilized during the harvesting process. While not specifically intended for tissue harvesting, these sets can assist in transferring fluids associated with the procedure. For preservation, OriGen Biomedical CryoPur® Solutions are intended for the cryopreservation of stem cells and blood components in a frozen state. These solutions can help maintain the viability of certain cellular components of harvested tissues for future use, though they are not specifically designed for preserving complex engineered tissues.
Phase 6: Storage and Transport
Engineered tissues often need to be stored or transported before application. Maintaining tissue viability during this phase is critical for successful outcomes.
OriGen Biomedical provides TissueVault™ Cell & Tissue Freezing Bag that may be beneficial in the storage process of engineered tissues, preserving their structure and function. Made from biocompatible EVO polymer, these bags are ideal for long-term cryogenic storage at temperatures as low as -196°C, including direct immersion in liquid nitrogen. They seal easily at 105°C with standard lab sealers and can be opened post-thaw by cutting below the seal. Despite their matte finish, the translucent design allows for easy visual inspection, making them a reliable choice for preserving solid tissues, bone, and other biological materials.
Phase 7: Application
The final phase involves the application of engineered tissues to the patient, either through surgical implantation or other medical procedures.
By providing high-quality products for each phase of the tissue engineering process, OriGen Biomedical plays a vital role in advancing regenerative medicine. Our comprehensive range of solutions supports researchers and clinicians in developing innovative tissue engineering therapies, ultimately improving patient outcomes and transforming healthcare.